Extraction Materials
-mortar and pestle
-extraction buffer-(contains the detergent Nonidet P-40)
-centrifuge
-centrifuge tube
-pipets
-grease pencil
-clean microtubes

Extraction Procedure
1- Place 3 grams of scuds and 4 mL of extraction buffer into chilled mortar and pestle and grind until a homogeneous suspension is formed.
2- Pour homogenate into a centrifuge tube a centrifuge for 5 minutes.
3- Pipet the supernatant into clean microtubes, label with grease pencil, and freeze.