Detection of LDH Isoenzymes Materials
-water bath
-thermometer
-gel staining dish
-LDH substrate
-dark bottle
-graduated cylinder

Detection of LDH Isoenzymes Procedure
1- Prepare LDH Substrate Solution: 1/6th substrate is dissolved in 16 mL of distilled water. LDH is stored in a dark bottle until use.
2- Add LDH solution to a gel stain dish containing the gel. Incubate in the dark for 1 hour in a 37°C water bath.
3- After incubation, rinse in water, examine under a light box, or store covered in water in refrigerator.