Research Paper On The Comparison Of The LDH Isoenzymes From Scuds From
Two Different Geographic Locations
Abstract
Two different populations of Gammarus fasciatus were studied to see if
their LDH isoenzymes were similar. Samples of Gammarus fasciatus were taken
from Bear's Grass Creek and Thompson Valley Creek, both in the Lower Eau
Claire Watershed in Eau Claire county. This study was to determine if geographic
isolation has effected the make-up of the two populations. Samples were
studied using gel electrophoresis, which separated proteins, and then stained
in a LDH stain. Twelve repetitions were done for each population. Stained
bands were measured and analyzed. Three results showed indications of a
difference between populations. The percent difference of electrophoretic
migrational distance between populations was: 24.44%, 12.5%, and 2.44%.
Researcher's Background
I, Anna Cousin, am currently a junior at Augusta High School in Wisconsin.
I researched on the gammarus fasciatus at the Augusta High School Science
Lab between June and September of 1996. Right away, I would like to recognize
and thank Mr. Paul Tweed, my Biology teacher as a mentor throughout my
research and web page setup.
I first became interested in this area of research when studying genetics
in Biology. I then did an independent study on DNA, proteins, and their
separation by gel electrophoresis, by following pre-made labs. I finally
prepared my question of individual study and carried out the research.
I presented my research and the Western Wisconsin/Upper Michigan Junior
Science and Humanities Symposium in September of 1996. I received sixth
place overall and am an alternate to attend nationals.
I designed this web page to make my research available to others. This
page is set up so that you can search my paper by section or view the complete
paper as a whole.
All materials and instructions can be obtained from:
Modern Biology, Inc.
P.O. Box 97
Dayton, Indiana 47941-0097
Tel: 1-800-733-6544
Do The LDH Isoenzyme Patterns Differ In
Scuds (Gammarus fasciatus) From Two
Different Geographic Locations?
Background Information
Hypothesis
Materials and Procedures
Analysis and Results
Conclusions
Bibilography
Map