Preparation of the Agarose Gels Materials
-electrophoresis buffer
-agarose
-casting tray
-glass slide
-casting comb
-masking tape
-plastic wrap
-hot plate
-250 mL beaker
-25 mL test tube
-glass stirring rod
-safety goggles
-test tube holder
-beaker tongs
-test tube tongs
-balance

Preparation of the Agarose Gels Procedure
1- Measure and pour 15 mL of electrophoresis buffer into test tube.
2- Weigh 0.18 grams of agarose on a balance and add to test tube.
3- Stir with glass stirring rod until the agarose forms a suspension.
4- Place test tube into a boiling water bath, stirring often, until mixture turns clear. Approximately 3-5 minutes.
5- Remove test tube and allow to cool for a minute.
6- Set up casting tray by placing glass slide in casting tray, placing comb in slots, and sealing ends with tape.
7- Pour cooled agarose into gel casting tray and cover with plastic wrap.
8- Leave small amount in test tube for testing application.
9- Refrigerate gel at least 15 minutes before using. Will keep in refrigerator no longer than three days.